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gfp-tagged human rab7a t22n  (Addgene inc)


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    Addgene inc gfp-tagged human rab7a t22n
    Gfp Tagged Human Rab7a T22n, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp-tagged human rab7a t22n/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    gfp-tagged human rab7a t22n - by Bioz Stars, 2026-02
    90/100 stars

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    Localization of exogenous ATP13A2 to intracellular acidic vesicles in cortical neurons. (A) Confocal fluorescence microscopy reveals the co-localization of exogenous V5-tagged human ATP13A2 with GFP-LC3 (autophagosomes), RFP-Rab5A (early endosomes), <t>GFP-Rab7A</t> (late endosomes) and LAMP1-RFP (lysosomes) in neuronal soma and processes. Exogenous GFP-tagged mouse ATP13A2 is specifically labeled by the ATP13A2 antibody (LMNR1). (B) ATP13A2-V5 localizes to punctate structures located upon βIII-tubulin-positive neuronal processes, whereas ATP13A2-GFP fails to co-localize with endogenous synaptophysin-1, a marker of synaptic vesicles. Cytofluorograms and correlation coefficients (Rcoloc) indicate the extent of co-localization between exogenous ATP13A2 and each marker. Confocal images are representative of at least two independent cultures. Scale bar: 10 µm.
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    Image Search Results


    Localization of exogenous ATP13A2 to intracellular acidic vesicles in cortical neurons. (A) Confocal fluorescence microscopy reveals the co-localization of exogenous V5-tagged human ATP13A2 with GFP-LC3 (autophagosomes), RFP-Rab5A (early endosomes), GFP-Rab7A (late endosomes) and LAMP1-RFP (lysosomes) in neuronal soma and processes. Exogenous GFP-tagged mouse ATP13A2 is specifically labeled by the ATP13A2 antibody (LMNR1). (B) ATP13A2-V5 localizes to punctate structures located upon βIII-tubulin-positive neuronal processes, whereas ATP13A2-GFP fails to co-localize with endogenous synaptophysin-1, a marker of synaptic vesicles. Cytofluorograms and correlation coefficients (Rcoloc) indicate the extent of co-localization between exogenous ATP13A2 and each marker. Confocal images are representative of at least two independent cultures. Scale bar: 10 µm.

    Journal: Human Molecular Genetics

    Article Title: PARK9-associated ATP13A2 localizes to intracellular acidic vesicles and regulates cation homeostasis and neuronal integrity

    doi: 10.1093/hmg/ddr606

    Figure Lengend Snippet: Localization of exogenous ATP13A2 to intracellular acidic vesicles in cortical neurons. (A) Confocal fluorescence microscopy reveals the co-localization of exogenous V5-tagged human ATP13A2 with GFP-LC3 (autophagosomes), RFP-Rab5A (early endosomes), GFP-Rab7A (late endosomes) and LAMP1-RFP (lysosomes) in neuronal soma and processes. Exogenous GFP-tagged mouse ATP13A2 is specifically labeled by the ATP13A2 antibody (LMNR1). (B) ATP13A2-V5 localizes to punctate structures located upon βIII-tubulin-positive neuronal processes, whereas ATP13A2-GFP fails to co-localize with endogenous synaptophysin-1, a marker of synaptic vesicles. Cytofluorograms and correlation coefficients (Rcoloc) indicate the extent of co-localization between exogenous ATP13A2 and each marker. Confocal images are representative of at least two independent cultures. Scale bar: 10 µm.

    Article Snippet: Expression plasmids containing human GFP-LC3B [plasmid no. 11546, ( 31 )], human RFP-Rab5A [plasmid no. 14437, ( 32 )], human GFP-Rab7A [plasmid no. 12605, ( 33 )] and rat LAMP1-RFP [plasmid no. 1817, ( 34 )] were obtained from Addgene.

    Techniques: Fluorescence, Microscopy, Labeling, Marker